ミクロレイヤー薄層クロマトグラフィーによる橙色および黄橙色系トマトのシスおよびトランス型カロテン類の定量

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献3件

書誌事項

タイトル別名
  • Determination of Cis and Trans Carotenes of Tangerine and Yellowish Tangerine Tomatoes by Micro-Thin-Layer Chromatography.
  • ミクロレイヤー薄層クロマトグラフィーによる橙色および黄橙色系トマトのシスおよびトランス型カロテン類の定量〔英文〕
  • ミクロレイヤー ハクソウ クロマトグラフィー ニ ヨル ダイダイイロ オヨビ

この論文をさがす

抄録

In the present study, cis and trans carotenes of recessive homozygote tt tomato lines were qualitatively and quantitatively analyzed by micro-thin-layer chromatography (m-TLC).<BR>1. Four cultivars and 4 lines were used: 3 tangerine tomatoes (RRtt), 'Jubilee', MM-Rt and RC-Rt; 2 yellowish tangerine tomatoes (rrtt), GJ-rt and MM-rt; 2 red tomatoes (RRTT), 'Moneymaker' and 'Red Cherry', and one yellow tomato (rrTT), 'Golden Queen'.<BR>2. The carotenes of RRtt and rrtt lines were separated into 11 spots (J1J10 and J5-2) by m-TLC on MgO: alumina: cellurose: CaSO4 (10 : 6 : 2 : 2, w/w/w/w), using the tech- nique of stepwise development of solvent mixtures, n-hexane: isopropanol: MeOH, from 100 : 2 : 0.2 (v/v/v) to 100 : 0 : 0 (v/v/v) (hexane alone).<BR>These 11 carotenes were identified to be the mixture phytoene and phytofluene (not separated), β-carotene, ζ-carotene, proneurosporene, pro-lycopene, pro-γ-carotene, trans- neurosporene, and four distinct lycopene compounds, poly-cis -lycopene (b), poly-cis - lycopene (a), neolycopene and trans -lycopene.<BR>3. When these carotenes were isomerized by iodine catalysis, the 11 spots were shifted to 6 spots. The chemical behavior of these isomerized carotenes was almost identical to that of the carotenes isolated from red tomatoes; namely, a mixture of phytoene and phy- tofluene and the trans forms of β-, ζ-and γ-carotene, neurosporene and lycopene.<BR>4. The amounts of carotenes developed on m-TLC were directly measured by chromato- scanner, using standard linear regression. Tangerine tomatoes (RRtt) contained 130 142 μg total carotene•g-1 fruit fresh weight which was about twice that of red toma- toes (RRTT, 7175 μg•g-1) and 5 to 10 times that of rrtt lines (1526μg•g-1). Yellow tomato (rrTT) contained about 4μg•g-1 total carotene.<BR>5. Recessive homozygote tt lines were characterized by a large number of cis form carotenes and a high concentration of cis and trans forms of ζ-carotenes (1944%), neurosporenes (1822%) and lycopenes (2048%). In addition, pro-γ-carotene was con- tained in a quantity similar to that of β-carotene, which suggests the presence of a new pathway leading to the formation of pro-γ-carotene in the biosynthesis of β-carotene.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 62 (3), 567-574, 1993

    一般社団法人　園芸学会

被引用文献 (3)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ