ササユリ球根のｉｎ　ｖｉｔｒｏにおける増殖に及ぼす液体通気培養条件の影響

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タイトル別名
  • Effects of Several Conditions in Aerated Liquid Culture on in vitro Multiplication of Bulbs of Lilium japonicum Thunb.
  • ササユリ キュウコン ノ in vitro ニ オケル ゾウショク ニ オヨボ

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To establish a practical in vitro mass-production system using bulb scales of Lilium japonicum, several cultural methods simplifying the process to enlarge the current system were compared. In addition, the effects of cultural conditions on the differentiation and growth of bulblets in aerated liquid culture were investigated.<BR>1. Four cultural methods : (a) liquid medium culture with polyester fiber support; (b) liquid medium culture with horizontal rotation; (c) liquid medium culture with aeration; and (d) capsule culture were tested. Among them, system (c) appeared to be the most practical.<BR>2. Bulblet differentiation was observed after 3 weeks of culture. The number of bulblets increased significantly 5 to 8 weeks after culture initiation but not thereafter. No increase in diameter of bulblets was observed after the 5th week of culture, indicating that additional nutrients should be supplemented to the medium after 8 weeks of culture for subsequent growth of the bulblets.<BR>3. Bulblet differentiation in aerated liquid culture was enhanced, using the medium with 3/4 strength MS macro-elemetns and 40g• liter-1 sucrose.<BR>4. Addition of NAA to the medium promoted bulblet differentiation and its optimal concentration was 0.01 mg liter-1. Addition of kinetin or ABA did not promote bulblet differentiation.<BR>5. Bulblets differentiate efficiently when aerated with ambient air (20.9%O2), whereas higher oxygen concentration was ineffective. Effects of temperature and light conditions on bulblet development in aerated liquid culture were similar to those of agar culture.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 62 (1), 197-205, 1993

    一般社団法人　園芸学会

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