Tracer Experiment Administering D-Glucose-U-<sup>14</sup>C and Shikimic Acid-G-<sup>14</sup>C as Related to Tyrosine Formation in Bamboo Shoots

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  • タケノコのチロシン生成に関与するD-グルコース-U-<sup>14</sup>C及びシキミ酸-G-<sup>14</sup>Cのトレーサー実験
  • タケノコのチロシン生成に関与するD-グルコースーU-14C及びシキミ類-G-14Cのトレーサー実験
  • タケノコ ノ チロシン セイセイ ニ カンヨスル D グルコース U 14C
  • Tracer Experiment Administering D-Glucose-U-14C and Shikimic Acid-G-14C as Related to Tyrosine Formation in Bamboo Shoots

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Abstract

D-glucose-U-14C and shikimic acid-G-14C known as good precursors for biosynthesis of aromatic compounds were fed to the tissue slices of both the apical and the basal sections of bamboo shoots for 3, 6, 9 and 21 hours at 30°C. After incubations, alcohol soluble substances were extracted with 70% methanol and then separated into organic acid, sugar and amino acid fractions by ion-exchange chromatography. The homogentisic acid fraction was collected from organic acid fraction by HPLC. In the meantime, lignin aldehyde was separated from alcohol insoluble residue by the method of alkaline nitrobenzene oxidation. The radioactivity incorporated into each compound was measured using a Packard Tri-Carb Scintillation spectrometer after adding toluene scintillator.<br>1. Glucose-U-14C was mainly incorporated into alcohol insoluble residue. The activity increased with the progress of incubation time and after 9 hours of incubation, it reached to 13.6% of the total radioactivity in the apical section and 52.5% in the basal section, but in which the activity of 14C incorporated into lignin aldehyde was very low. By determining the activity incorporated into each amino acid from glucose, it was found that the radioactivity was detected in almost all of amino acids, and also that the highest activity was found in alanine among amino acids in both sections. In the meantime, the radioactivity incorporated into tyrosine or phenylalanine was several percent in both sections.<br>2. Shikimic acid-G-14C was steadily incorporated into the tissues during incubation and was rapidly converted to aromatic amino acids (tyrosine and phenylalanine). The incorporation of shikimic acid into lignin aldehyde increased during incubation and the activity was higher in the basal section than in the apical section. On determining the amount of homogentisic acid, it was found that the change from shikimic acid to homogentisic acid was very low in both sections.

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