培養容器内のキクを用いたキク白さび病菌の培養

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 参考文献6件

書誌事項

タイトル別名
  • Incubation of Puccinia horiana P.H. Using Chrysanthemum Plants Cultured In Vitro.
  • バイヨウ ヨウキ ナイ ノ キク オ モチイタ キク シロサビ ビョウキン ノ バイヨウ

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説明

To propagate and maintain the pathogen, Puccinia horiana P. H. as the inoculum of white rust, sterile chrysanthemum plants cultured in vitro were infected. 1. Chrysanthemum plants, growing in vitro, were infected with spores of Puccinia horiana without cross contamination by fixing the sporulated leaf from an infected plant grown in a greenhouse above leaves of sterile receptor plants. 2. Puccinia horiana was subcultured by placing leaves with telia to the backside of the cap of glass culture box containing sterile chrysanthemum plants. 3. Puccinia horiana incubated on the plants can be preserved at low temperature for 4 months. 4. The pathogenicity of Puccinia horiana, subcultured on the plants grown in vitro onto plants in a greenhouse, was maintained for at least 14 months after the primary incubation.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 69 (6), 767-769, 2000

    一般社団法人　園芸学会

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