胚珠培養によるSpiraea thunbergii Sieb.とSpiraea japonica L.との種間雑種の作出

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献4件 参考文献25件

書誌事項

タイトル別名
  • Interspecific Hybrids between Spiraea thunbergii Sieb. ex Blume. and S. japonica L. fil. via Ovule Culture.
  • はい珠培養によるＳｐｉｒａｅａ　ｔｈｕｎｂｅｒｇｉｉ　Ｓｉｅｂ．とＳｐｉｒａｅａ　ｊａｐｏｎｉｃａ　Ｌ．との種間雑種の作出
  • ハイガ バイヨウ ニ ヨル Spiraea thunbergii Sieb ト Spiraea japonica L ト ノ シュ カン ザッシュ ノ サクシュツ

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Reciprocal crosses between Spiraea thunbergii Sieb. ex Blume. and S. japonica L. fil. were carried out, to incorporate red color to S. thunbergii. In vitro germination rate of the stored pollen, germination of pollen grains on the stigma, growth of the pollen tubes, and the development of ovule were investigated, in addition ovule culture was tried to obtain interspecific hybrids. 1. Since the flowering time of both species differed, pollen grains of both species were stored dry at -30°C, 5°C, and 20°C. Only the pollen of both species stored at -30°C for 360 days remained viable for crossing. 2. Pollen grains of S. japonica, stored at -30°C, germinated well on the stigma of S. thunbergii. The pollen tubes elongated rapidly to the ovary as the developing ovules were observed 10 days after pollination. Stored pollens of S. thunbergii germinated well on the stigma of S. japonica, but whether the pollen tubes reached the ovary it was not confirmed. 3. In the interspecific crosses between S. thunbergii (♀) and S. japonica (♂), enlarged ovules excised 16 days after pollination were cultured in 1/2MS medium containing 30g·liter-1 sucrose and 8g·lite-1 agar. The resulting plantlets seem to be true hybrids between S. thunbergii and S. japonica., based on several morphological characteristics and the RAPD banding patterns which exhibited the same bands as the parents.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 70 (6), 767-773, 2001

    一般社団法人　園芸学会

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