Effects of Nitrogen Source, Phosphate and Sulfate in Liquid-Shaking Culture on the Growth of Miniature Bulbs of Lilium japonicum Thunb.
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- Haruki Kazuhisa
- Faculty of Life and Environmental Science, Shimane University
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- Yamada Kazuto
- Shimane Agricultural Experiment Station
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- Hosokil Takashi
- Faculty of Life and Environmental Science, Shimane University
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- Ohta Katsumi
- Faculty of Life and Environmental Science, Shimane University
Bibliographic Information
- Other Title
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- 液体振とう培養におけるササユリ球根の生育に及ぼす窒素源,リン酸イオンおよび硫酸イオンの影響
- エキタイ シン ト ウ バイヨウ ニ オケル ササユリ キュウコン ノ セイイ
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Abstract
For liquid-shaking culture of bulbs of Liliurn japonicum Thunb., we analyzed the concentration changes in macro-inorganic ions in the culture media to clarify their relationships with bulb growth.<BR>1. Inorganic ions, SO42-, NH4+, and especially H2PO4- were consumed in large quantities by bulbs during culture, whereas the consumption of K+, Mg2+, and Ca2+ was very slow.<BR>2. The optimum concentration of NH4+ for bulb enlargement was 10-20 mM. The application of NO3- alone or NH4+ higher than 30 mM as nitrogen source retarded the bulb growth. Root elongation was promoted by application of NO3- as the sole nitrogen source.<BR>3. Root growth was poor in the medium containing a high ratio of NH4+ /NO3-. Application of glutamine instead of NH4+ promoted root growth.<BR>4. The in vitro rooted bulbs sprouted and bolted abundantly after planting in the soil. Based on the above results, bulbs should be cultured first in a medium containing NH4+ for bulb enlargement and then transferred to a NH4+ free medium for root development. These treatments improved shoot growth after transplanting.<BR>5. Although the absorption rate of H2PO4- from the medium with the high concen-tration of NaH2PO4 was higher than that from the medium with the low concentration, bulb enlargement was not promoted.<BR>6. High concentration of SO42- in the medium inhibited bule growth.
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 65 (2), 387-396, 1996
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206303870592
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- NII Article ID
- 10006100289
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- NII Book ID
- AN00025257
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- COI
- 1:CAS:528:DyaK28XlvV2rsbg%3D
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- ISSN
- 1880358X
- 00137626
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- NDL BIB ID
- 4029204
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed