Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process

DOI IR Web Site PubMed 1 Citations 22 References Open Access
  • Asai Emiko
    Department of Plastic and Reconstructive Surgery, Fukushima Medical University
  • Yamamoto Masaya
    Department of Anatomy and Histology, Fukushima Medical University
  • Ueda Kazuki
    Department of Plastic and Reconstructive Surgery, Fukushima Medical University
  • Waguri Satoshi
    Department of Anatomy and Histology, Fukushima Medical University

Search this article

Description

<p>To investigate the possible implications of autophagy, one of the degradation pathways induced by metabolic stress, in the dynamic reconstructive process of wound healing, the appearance and changes of punctate structures for microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, were examined in a rat skin wound healing model. Although the ratio of LC3-II/LC3-I in Western blotting was not evidently changed during the wound healing process, LC3-positive dots were clearly observed in fibroblasts and myofibroblasts, and occasionally in macrophages, by immunohistofluorescence microscopy. Some of the LC3-positive dots were colocalized with Atg16L signal, an isolation membrane marker, and electron microscopy revealed the presence of typical autophagosomes in fibroblasts near the margin of the wound. The number of LC3-positive dots per fibroblast increased during the later period of the proliferation phase, and interestingly, it was higher in the margin than the center of the wound. It was also high in the periwound skin area. These results suggest that drastic functional changes in fibroblasts during wound healing process are accompanied by the alteration of the autophagy-lysosomal degradation system.</p>

Journal

Citations (1)*help

See more

References(22)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top