Comparison of precipitation methods for quantification of high-density lipoprotein cholesterol.

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  • IMAMURA Chisa
    Department of Clinical Pathology, National Cancer Center Hospital
  • WADA Yoshiko
    Department of Clinical Pathology, National Cancer Center Hospital
  • ARAKI Eiji
    Department of Clinical Pathology, National Cancer Center Hospital

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  • 結合沈殿法を用いる血清高密度‐リポタンパクコレステロール測定法の比較

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Abstract

We evaluated the polyethylene glycol-dextran sulfate-MgCl2 method (A) incomparison with phosphotungstate-MgCl2 method (B), heparin-CaCl2, NiCl2 method (C) and polyethylene glycol method (D) for the quantification of high-density lipoprotein (HDL)-cholesterol.<br>Methods were compared for precision, effectiveness of lipoprotein sedimentation. Within run precision of method A as indicated by CV ranged from 0.9 to 1.1%. Between day CVs were 3.7% and 3.4% for 33.8mg/dl and 89.5mg/dl, respectively. The supernatants obtained with method A, B, C, and D for normal sera were proved to be devoid of β- and pre-β-lipoproteins as judged by agarose electrophoresis with lipid and cholesterol stain. As for the hyperlipidemic and/or jaundiced sera, containing slow-α-fraction, method D failed to precipitate this abnormal lipoprotein and gave higher values of HDL-cholesterol compared with method A, in that abnormal lipoproteins are precipitated completely. No interference by bilirubin, hemoglobin or turbidity was observed for method A. Values obtained with precipitation technique and those with agarose electrophoresis and enzymatic color development for cholesterol correlated well. This precipitation technique, polyethylene glycoldextran sulfate-MgCl2 method, is appropriate for routine clinical laboratory use in the assay of HDL-cholesterol in various pathological status of lipoprotein metabolism.

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