Unification of the Genera Serpulina and Brachyspira,and Proposals of Brachyspira hyodysenteriae Comb.Nov.,Brachyspira innocens Comb.Nov.and Brachyspira pilosicoli Comb.Nov
-
- Ochiai Sachiyo
- School of Agriculture, Ibaraki University
-
- Adachi Yoshikazu
- School of Agriculture, Ibaraki University
-
- Mori Katsumi
- National Food Research Institute
書誌事項
- タイトル別名
-
- Unification of the Genera <i>Serpulina</i> and <i>Brachyspira</i>, and Proposals of <i>Brachyspira hyodysenteriae</i> Comb. Nov., <i>Brachyspira innocens</i> Comb. Nov. and <i>Brachyspira pilosicoli</i> Comb. Nov.
- Unification of the Genera Serpulina and
この論文をさがす
抄録
The phylogenetic positions of Serpulina hyodysenteriae, Serpulina innocens, Serpulina pilosicoli and Brachyspira aalborgi were studied. Complete 16S ribosomal DNA sequences of these three species and B. aalborgi revealed that their 16S rDNA sequences were related more than 96.0%. The mol% guanine plus cytosine (G+C) of B. aalborgi DNA was 27.1, and was similar to those of the 3 members of the genus Serpulina. The homologous rates using 32P-labeled B. aalborgi chromosome DNA in DNA-DNA reassociation tests were 22.0% to S. hyodysenteriae, 19.1% to S. innocens and 17.2% to S. pilosicoli. Therefore, we propose to transfer the three species of the genus Serpulina to the genus Brachyspira. Descriptions of Brachyspira hyodysenteriae comb. nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov., and an emended description of B. aalborgi are given. Phenotypic characteristics of the 4 members of the genus Brachyspira were also studied. They fermented fructose, galactose, glucose, lactose, maltose, mannose, raffinose and trehalose; however, B. aalborgi did not ferment raffinose. All of them hydrolyzed esculin but did not produce indole except for B. hyodysenteriae. The protein profile of B. aalborgi was different from those of the four strains of B. hyodysenteriae, B. innocens and B. pilosicoli, but the heavy bands with molecular sizes of 49.4 and 52.3kDa of B. aalborgi were quite similar to those of B. innocens in the points of quantity and molecular size. In immunoblotting tests, B. aalborgi reacted well with anti-B. innocens and B. pilosicoli sera, but reacted weakly with anti-B. hyodysenteriae serum. Only one heavy band and several faint bands were revealed by the reaction between B. aalborgi and anti-B. hyodysenteriae serum, and the heavy band was common among these strains.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 41 (6), 445-452, 1997
財団法人 学会誌刊行センター
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206318422784
-
- NII論文ID
- 10004896949
-
- NII書誌ID
- AA00738350
-
- COI
- 1:CAS:528:DyaK2sXkvVaktr4%3D
-
- ISSN
- 13480421
- 03855600
- http://id.crossref.org/issn/03855600
-
- NDL書誌ID
- 4231140
-
- PubMed
- 9251055
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可