TWO REAGENTS FOR FLUORESCENT ANTIBODY TECHNIQUE AND THE VISUALIZATION OF THE VIRAL ANTIGEN SYNTHESIZED IN EHRLICH ASCITES TUMOR CELLS INFECTED WITH ED VIRUS (STUDIES ON VIRAL ONCOLYSIS 4.)
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- ITO MICHIO
- Institute for Infectious Diseases, University of Tokyo
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- NISHIOKA KUSUYA
- Institute for Infectious Diseases, University of Tokyo
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説明
1) A fluorescent antibody technique using 1-dimethylaminonaphthalene 5-sul-phonylchloride and Xylene Red B has been described. The conjugation procedure for each dye with protein is simple without any deterioration of the antibody activity.2) The quality of each conjugate was determined by use of a Beckman spectro-photometer and spectrofluorometer. 3) The viral antigen synthesized in Ehrlich ascites tumor cells infected with ED virus was distinctly visualized by staining of each conjugate, and the specificity of fluorescent staining of antigen was confirmed with the systematic control tests. <br>XRB-conjugated protein was preferable for the detection of antigen, because it showed brilliant orange fluorescence and could be clearly discriminated from the auto-fluorescene of cells. 4) The viral antigen was detected in the cytoplasm of the infected cells within 6 hous after virus challenge, and increased continuously until a maximum was reached at 24 hours. 5) The observation by mean of fluorescent antibody technique confirms the the previous results obtained by immunochemical and cell-fractionation studies.
収録刊行物
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- Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ
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Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ 3 (1), 71-83, 1959
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