Studies on antigenic activities of yeasts-5-Effect of α-mannosidase digestion on the immunochemical properties of the mannan of Saccharomyces cerevisiae

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  • SUNAYAMA Hiroyoshi
    The Second Department of Hygienic Chemistrh, Tohoku College of Pharmacy
  • SUZUKI Shigeo
    The Second Department of Hygienic Chemistrh, Tohoku College of Pharmacy

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タイトル別名
  • Studies on the Antigenic Activities of Yeasts1
  • Studies on antigenic activities of yeasts 5 Effect of アルファ-mannosidase digestion on the immunochemical properties of the mannan of Saccharomyces cerevisiae
  • Studies on the Antigenic Activities of Yeasts
  • V. Effect of α-mannosidase Digestion on the ImmunochemicalProperties of the Mannan of Saccharomyces cerevisiae

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In order to provide further information on the chemical nature of the antigenicdeterminants of the mannan of Saccharomyces cerevisiae, the mannan was digested by Arthrobacter α-mannosidase, and 9, 21, 35, 59 and 62%-partially degraded mannanswere prepared in the present study. Acetolysis of each degraded mannan showed thatonly a small amount of the tetrasaccharide was detectable in the 35%-digested mannan, whereas the predominant product of the 59 and 62%-digested mannan was mannose.The result of a quantitative precipitation reaction with the degraded mannans showedthat the precipitation activities were partially or completely destroyed by the action ofthe enzyme. The lack of the tetrasaccharide moieties of the mannan were noticeableby a decrease in the precipitating ability. It was observed that the decreasing ratio ofeither the maximum amount of the antibody N precipitable by the mannan or percent degradation of the mannan were essentially equal and yielded nearly a straightrelationship between 0 and 2.0 hr digestion. However, the 59 and 62%-digested mannans, containing trace amounts of di- and trisaccharides in the branching parts, showedno significant antigenic activities. Furthermore, the molar ratio of the tetrasacchariderelative to the trisaccharide also gradually decreased. These observations confirm thatthe tetrasaccharide moiety, Manα1→3Manα1→2Manα1→2Man, plays an importantrole as the antigenic determinant. The core mannan moiety completely lost both theprecipitating ability and inhibitory activity in ranges employed up to 1500μg. Thesefindings offer a direct proof that the core mannan moiety of mannan is not responsiblefor antigenic activity, and functions merely as the "gcarrier" of the antigenic determinantswhich dominate the immunological specificity.

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