Electron Microscopic Studies on the Outer Layers of <i>Staphylococcus aureus</i> Using a Lytic Enzyme from <i>Flavobacterium</i>
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- FUTAI Masamitsu
- Faculty of Pharmaceutical Sciences, University of Tokyo
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- OKABAYASHI Kinji
- Faculty of Pharmaceutical Sciences, University of Tokyo
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- MIZUNO Den'ichi
- Faculty of Pharmaceutical Sciences, University of Tokyo
Bibliographic Information
- Other Title
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- Electron microscopic studies on the outer layers of Staphylococcus aureus using a lytic enzyme from Flavobacterium
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Description
The structure of the outer layers (cell wall and membrane) of Staphylococcus aureus was studied by electron microscope using a bacteriolytic enzyme from Flavobacterium sp. called the L-11 enzyme. Comparative studies on the morphology of bacteria before and after treatment with this enzyme and cell wall and membrane fractions obtained from bacteria after the enzyme treatment led to the following conclusions. (1) The cell wall of S. aureus is composed of morphologically distinct two layers which are both susceptible to the L-11 enzyme. (2) Between the cell wall and membrane, there is an electron opaque region which could not be stained using any of the methods tested. (3) Before treatment of bacteria with the enzyme the cell membrane could not be seen clcarly. However, after enzyme treatment the membrane was clearly seen. (4) The infolding of the inner layer of the cell wall, forming a structure like a mesosome, was liberated by extensive enzyme treatment.
Journal
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- Japanese Journal of Microbiology
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Japanese Journal of Microbiology 16 (5), 341-350, 1972
Center For Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206319448064
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- NII Article ID
- 130004237229
- 40018694192
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- NII Book ID
- AA00246855
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- COI
- 1:CAS:528:DyaE3sXis1SrsQ%3D%3D
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- NDL BIB ID
- 7674508
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- ISSN
- 00215139
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- PubMed
- 4539618
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed