Chemical and Biological Properties of the Purified O Antigen of Bordetella pertussis
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- NAKASE Yasukiyo
- Department of Bacteriology, The Kitasato Institute
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- TATEISI Masayoshi
- Department of Bacteriology, The Kitasato Institute
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- SEKIYA Kachiko
- Department of Bacteriology, The Kitasato Institute
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- KASUGA Tadayoshi
- Department of Bacteriology, The Kitasato Institute
Bibliographic Information
- Other Title
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- Chemical and Biological Properties of the Purified O Antigen of <i>Bordetella pertussis</i>
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Abstract
A cell wall fraction obtained by sonic oscillation, yielded a Bordetella pertussis O antigen which possessed the ability to both adsorb O agglutinins and to produce the O agglutinins in animals. It was purified by a phenol water extraction and centrifugation method. Physico-chemical and biological properties of the O antigen were similar to that of the so-called endotoxic lipopolysaccharide. It contained 2.6% nitrogen, 1.2-1.5% phosphorus, 9.0-11.0% heptose, 0.3% 2-keto-3-deoxyoctonate (KDO), 13.3-13.4% as total carbohydrate, 16-16.5% hexosamine and 25-32% lipid. Homogeneity of the purified O antigen was confirmed by ultracentrifugation, electronmicrograph and geldiffusion tests. It possessed significant pyrogenicity, adjuvant effect on antibody produc-tion, and toxicity to animals, but not protective antigenicity.
Journal
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- Japanese Journal of Microbiology
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Japanese Journal of Microbiology 14 (1), 1-8, 1970
Center For Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206319936000
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- NII Article ID
- 130004237095
- 40018694593
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- NII Book ID
- AA00246855
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- COI
- 1:CAS:528:DyaE3cXks1CgsbY%3D
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- NDL BIB ID
- 8511673
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- ISSN
- 00215139
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- PubMed
- 4313572
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed
