Determination of Folate Derivatives in Rat Tissues during Folate Deficiency.

DOI Web Site PubMed 3 Citations 28 References
  • NAKATA Rieko
    Department of Food Science and Nutrition, Nara Women's University

Search this article

Abstract

A method for the sensitive and specific determination of folate derivatives was developed. The method involves hydrolysis by γ-glutamyl hydrolase and high-performance liquid chromatography with electrochemical detection. The method was applied to measure the change in the level of folate derivatives in the liver, kidney, spleen and brain of rats during folate deficiency. 5, 6, 7, 8-Tetrahydrofolic acid was the major folate derivative in the liver, kidney, spleen and brain. Total concentration of folate derivatives decreased from the second week of folate deficiency in the liver, kidney, spleen and brain followed by anemia, which ap-peared at the fifth week. The level of 5, 6, 7, 8-tetrahydrofolic acid in the brain did not change during folate deficiency, but it significantly decreased in the liver, kidney and spleen.

Journal

Citations (3)*help

See more

References(28)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top