EVALUATION OF THE THERAPEUTIC EFFECT OF KANAMYCIN (KM) AND STREPTOMYCIN (SM) IN THE TREATMENT OF MURINE LEPROSY IN RATS

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  • 鼠癩菌感染ダイコクネズミに対するKanamycin(KM)及びStreptomycin(SM)の治療効果判定について
  • ネズミライキン カンセン ダイコクネズミ ニ タイスル Kanamycin KM オヨビ Streptomycin SM ノ チリョウ コウカ ハンテイ ニ ツイテ

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Abstract

White rats infected with the K.UM.AMO TO-strain of M. leprae murium were divided into 3 groups ; the first was treated with 5 mg of KM daily, the second with the same dose of SM and the third was remained as the non-treated control. After the treatment of 3.5 to 4.5 months animals were autopsied and the nodules at the site of inoculation were removed, weighed and emulsified. The emulsions were diluted in decimal system. Two smears were prepared from every dilution degree of the emulsion quantitatively and were stained with victoriablue simultaneous staining and malachitegreen-fuchsin staining respe-ctively for the counting of the number of bacilli in 0.1 ml, and then total number of bacilli per leproma was calculated. After that, 0.5 ml of 11500 dilution of the emulsions of nodules were inoculated subcutaneously into normal healthy rats and the development of nodule at the site of inoculaton was observed.<br>In the treated groups the weight of nodules were markedly lighter and the total numbers of bcilli per nodules were also smaller than the control group. In the control group, majority of the bacilli stained to malachitegreen and was typical rod shaped form, whereas in the treated groups they could not be stained by malachitegreen and represent-ed granular or beaded form. Such a difference was more evident in the KM group than in the SM group.<br>Leprosy nodules which were produced by inoculation of the emulsions of the nodules from treated animals appeared much later than those produced by the emulsion from control animal.<br>From these results it is clear that the administration of KM or SM is very effective in the treatment of murine leprosy in rats. Further, malachitegreen-fuchsin staining seems to be usable in the evaluation of the therapeutic effect of medicaments enabling to show qualitative differences of murine leprosy bacilli between the treated and the untreated animals.

Journal

  • Repura

    Repura 28 (3), 80-84, 1959

    JAPANESE LEPROSY ASSOCIATION

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