鼠癩菌感染マウスより分離された小川氏抗酸菌について

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書誌事項

タイトル別名
  • Ogawa's Bacillus: Slow Growing Mycobacteria Isolated from Mice Previously Infected with Murine Leprosy Bacillus
  • 試験管内培養と動物接種
  • シケン カンナイ バイヨウ ト ドウブツ セッシュ
  • (第1報) 試験管内培養と動物接種
  • I. In Vitro Cultivation and Animal Inoculation

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説明

In 1969, Ogawa and Motomura1) isolated slow growing acid-fast bacilli from mice previously inoculated with the Hawaiian strain of Mycobacterium lepraemurium. The 4th subcultures of the organisms, designated here as strain HI of Ogawa's bacillus, were given through the courtesy of Dr. Ogawa and submitted to the present experiment in which comparative studies with other known strains of slow growing mycobacteria were carried out.<br>Growth characteristics in vitro: When heavily transferred by loop (Table 1), the organisms grew very slowly on Ogawa's 1% egg yolk medium1) but not on the 1% egg medium4), the egg yolk medium deprived of glycerol, or the Morrison6) and Smith7) agar media containing mycobactin. The addition of heat-killed M. phlei cells to the egg yolk medium did not enhance the growth of the organisms. Small inocula from the filtered bacterial suspension, 0.01mg to 0.3mg (moist weight), yielded isolated colonies on the egg yolk medium; growth became barely visible to the naked eye after approximately 40 weeks' incubation at 37° (Table 2).<br>Animal test: Male mice of dd-Y strain, weighing 20 to 22g, were inoculated intravenously with an amount of 0.2 or 0.02mg of the 4th subculture and utopsied 5 to 10 months later. As shown in Table 3, the organisms gave rise to a progressive murine leprosy-like disease with severe lesions involving liver, spleen, and skin; the lungs were less severely affected. Most of the smears from these tissues revealed vast number of acid-fast bacilli. For isolation weighed tissue was ground in a mortar and treated with 5 to 10 volumes of 1 per cent sodium hydroxide; 0.1-ml amounts (20 to 10mg of tissue) of the resulting suspension were pipetted into Ogawa's egg yolk and egg slants. Of a total of 59 specimens inoculated, 35 gave positive cultures on the egg yolk medium, 19 negative and 5 contaminated. No positive cultures were obtained with the egg medium. All of the cultures recovered were identified with the organisms employed for injection.<br>It seems reasonable to conclude that strain HI is very characteristic in vitro and in experimental animals, and definitely distinguishable from other known culturable mycobacteria. Some similarities between Ogawa's bacillus and murine leprosy bacillus has briefly discussed.

収録刊行物

  • レプラ

    レプラ 41 (3), 127-136, 1972

    日本ハンセン病学会

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