Effects of Microaerobic and Iron-deficient Cultivation on the Chemical constitution of Mycobacterium smegmatis

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  • 鉄欠乏下ならびに低酸素分圧下培養のスメグマ菌構成成分に与える影響について
  • テツ ケツボウ カ ナラビニ テイサンソ ブンアツ カ バイヨウ ノ スメグマキン コウセイ セイブン ニ アタエル エイキョウ ニ ツイテ

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It has been known that several strains of mycobactetia grown in vivo are considerably different from the same strain of mycobacteria grown in vitro in their biochemical properties such as cytochrome content or lipid constitution etc. As an approach to elucidate the reason why such differences do occur between these mycobacteria, attempts were made to cultivate M. smegmatis, Ju-cho strain, on an iron-deficient synthetic medium under a micro-aerobic condition, then effects of such culturing conditions on the chemical constitution of the micro-organism were investigated. Consequently, it was found that this strain of mycobacterium could be grown, though barely, under such culturing conditions, and cells thus obtained showed significant increases in total nitrogen content, RNA-phosphorus content, chloroform-soluble lipid content and constitutive ratio of stearic acid and also significant decreases in DNA-phosphorus content, ether-ethanol-soluble lipid content and constitutive ratio of oleic acid as compared with cells of the same strain grown aerobically on Sauton's medium. These changes, however, in the chemical constitution of M. smegmatis were not so drastic as those found in mycobacteria grown in vivo. From results thus obained and some other reports, an assumption was made concerning the reason of biochemical deviation occured in mycobacteria grown in vivo.

Journal

  • Repura

    Repura 40 (3), 160-164, 1971

    JAPANESE LEPROSY ASSOCIATION

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