Multiplication of Mycobacterium leprae in the Foot-pads of Mice

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  • マウス足底部におけるM.lepraeの増殖
  • マウス アシゾコブ ニ オケル M.leprae ノ ゾウショク

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Abstract

The transmission of M. leprae into experimental animals had been studyed by means of the inoculation into foot-pads of mice since 1964, and it was indicated that M, leprae could multiply successfully in the tissue of mice foot-pads, as reported by C.C. Shepard. <br>In our laboratory, M. leprae was isolated from leproma of Japanese leprosy patients of lepromatous type by using the mice of some Japanese strains. At present, 9 strains of M. leprae are maintained in more than fourth generation and 7 strains of M. leprae are kept under investigation. In 9 strains of M. leprae, 7 strains are isolated from untreated patients and 2 strains are isolated from treated patients. <br>Very easy method was used for the counting of acid-fast bacilli; The emulsion of tissue of mouse foot-pad was made by using the pestle arid mortar, and one drop of this emulsion was located on each slide-glass by using the pestle. The number of acid- fast bacilli in this smear was counted microscopically and the average bacillary count could be obtained.<br>In this studies, it was found that the multiplication of bacilli was indicated by the appearance of globi-like findings gathered each other with bacilli and that this growth was limited in the foot-pad of mouse. The transmission of M. leprae into mice of next generation may be tried at about 6 months after the infection and the bacillary number of more than 104 in each foot-pad of mouse may be required in subtransmission, because many killed bacilli might be contained in this emulsion of tissue of mouse foot-pad.<br>The multiplication of M. leprae was increased by the administration of Cortisone, but this could not be influenced by the injection with Mitomycin C, 6-Mercaptopurine and 2-Mercaptoethanol. However, the subcutaneous injection of Adjuvant into the foot-pads of mice inhibited the multiplication of M. leprae.

Journal

  • Repura

    Repura 37 (1), 15-24, 1968

    JAPANESE LEPROSY ASSOCIATION

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