Induction of Pre‐Early Nuclear Antigen(s) in HEL Cells Infected with Human Cytomegalovirus
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- TANAKA Shigeaki
- Department of Molecular Biology, Tokai University, School of Medicine
書誌事項
- タイトル別名
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- Induction of Pre-Early Nuclear Antigen (s) in HEL Cells Infected with Human Cytomegalovirus
- Induction of Pre Early Nuclear Antigen
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説明
Human cytomegalovirus (HCMV) -specific nuclear antigen could be detected within 1 hr after infection in human embryo lung cells by the anticomplement immunofluorescence (ACIF) test. This antigen has been named the pre-early nuclear antigen (PENA) in this paper. Serum absorption tests suggested that PENA is immunologically different from the early antigen and the major nuclear inclusion antigens detected by the indirect immunofluorescence test before and after viral DNA replication, respectively. PENA-forming ability of the virus corresponded to its plaque forming ability. PENA formation was not affected by phosphonoacetate but was inhibited by the addition of inhibitors of RNA and protein syntheses or by UV-irradiation of infecting virus, suggesting that the for mation of PENA depends on the expression of infecting virus gene functions. Virus-specific proteins were isolated by indirect immunoprecipitation from HCMV-infected cells exposed to 35S-methionine. SDS-polyacrylamide gel electrophoresis of the immunoprecipitate showed that at least two species of virus-specific polypeptides with molecular weights of 70, 000 and 30, 000 were synthesized de novo within 3 hr after infection.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 23 (4), 263-271, 1979
財団法人 学会誌刊行センター
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詳細情報 詳細情報について
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- CRID
- 1390001206322662912
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- NII論文ID
- 130003482788
- 40005319057
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- NII書誌ID
- AA00738350
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- COI
- 1:CAS:528:DyaE1MXkvVyru7c%3D
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- ISSN
- 13480421
- 03855600
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- NDL書誌ID
- 2083848
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- PubMed
- 224287
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可