<i>Lmx-1b</i> and <i>Wnt-7a</i> expression in axolotl limb during development and regeneration
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- SHIMOKAWA Takashi
- Division of Anatomy II, Kanazawa Medical University
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- YASUTAKA Satoru
- Division of Anatomy II, Kanazawa Medical University
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- KOMINAMI Rieko
- Division of Anatomy II, Kanazawa Medical University
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- SHINOHARA Harumichi
- Division of Anatomy II, Kanazawa Medical University
Bibliographic Information
- Other Title
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- Lmx-1b and Wnt-7a expression in axolotl limb during development and regeneration
Description
Axolotls (Ambystoma mexicanum) have the ability to regenerate amputated limbs throughout their life span. During limb regeneration as well as development, undifferentiated cells in the blastema acquire positional information to reproduce the original pattern along three cardinal limb axes: anteroposterior, proximodistal and dorsoventral. In the present study, we attempted to understand the molecular mechanism involved in patterning of axolotl limb development and regeneration along the dorsoventral (DV) axis. We cloned axolotl Lmx-1b and Wnt-7a, and investigated the expression pattern of these genes in developing and regenerating limbs. In axolotl, unlike in amniotes, Wnt-7a was expressed in a diffuse manner throughout both developing limb bud and regenerating limb blastema. Lmx-1b expression was observed at the dorsal mesenchyme in the developing and regenerating limbs. On the basis of the expression patterns of Lmx-1b and Wnt-7a, it was difficult to identify the interaction between these two genes as reported in amniotes in previous studies. Possibly, with regard to Lmx-1b expression, a Wnt-7a-independent mechanism may exist in axolotl limb development and regeneration.
Journal
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- Okajimas Folia Anatomica Japonica
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Okajimas Folia Anatomica Japonica 89 (4), 119-124, 2013
Editorial Board of Okajimas Folia Anatomica Japonica
