Multiplication of Mycobacterium lepraemurium in Cell-free Liquid Medium

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Other Title
  • 無細胞液体培地における鼠癩菌の増菌
  • ム サイボウ エキタイ バイチ ニ オケル ネズミライキン ノ ゾウキン
  • 無細胞液体培地における鼠癩菌の増菌-11-ND-5培地の開発
  • ム サイボウ エキタイ バイチ ニ オケル ネズミライキン ノ ゾウキン 11
  • 第11報 ND-5培地の開発
  • 11. Establishment of the ND-5 Medium

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Description

In order to improve the NC-5 medium, the Dubos medium (pH 7.3) was used as a basal medium, instead of Kirchner medium. The complete medium thus prepared is referred to as ND-5 medium. In this medium, Mycobacterium lepraemurium quickly multiplies in the form of binary fission without an extraordinary elongation. Possible generation times could be calculated by repeated experiments as 1.4-2.6 days. A slightly degenerative changes in the cells during prolonged cultivation was observed by electron microscopy. This medium has some advantages for inhibiting other bacterial contaminations. Serial subcultivation is not tested yet.

Journal

  • Repura

    Repura 45 (4), 211-216, 1976

    JAPANESE LEPROSY ASSOCIATION

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