ISOTOPIC STUDIES ON 2′-DEOXYINOSINE-8-C<sup>14</sup> FORMATION FROM INOSINE-8-C<sup>14</sup> BY WASHED CELL SUSPENTION OF VITAMIN B<sup>12</sup>-REQUIRING STRAIN OF <i>LACTOBACILLUS</i>

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  • MIYAZAWA EIKO
    Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • MORINAGA KOTARO
    Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • MAEKAWA AKIO
    Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • SUZUKI TAKAO
    Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture
  • SAHASHI YOSHIKAZU
    Laboratory of Biochemistry and Nutrition, Department of Agricultural Chemistry, Tokyo University of Agriculture

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  • Isotopic studies on 2'-deoxyinosine-8-C14 formation from inosine-8-C14 by washed cell suspention of vitamin B12-requiring strain of Lactobacillus leichmannii
  • ISOTOPIC STUDIES ON 2′-DEOXYINOSINE-8-C14 FORMATION FROM INOSINE-8-C14 BY WASHED CELL SUSPENTION OF VITAMIN B12-REQUIRING STRAIN OF LACTOBACILLUS

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1. Isolation and confirmation of 2′-deoxyinosine and related compounds which appeared in the reaction mixture were made by two-dimensional ascending paper chromatography, and each spot was applied to bioautography, autoradiography and chromatoscanning. The recoveries of the standard compounds previously added to the reaction mixture were satisfactory.<br>2. Formation of 2′-deoxyinosine-8-C14 from inosine-8-C14 In the presence of vitamin B12 was tested by two-dimensional paper chromatography, and paper chromatoscanning, and the labeled deoxyinosine was detected in the amount of 8% of the added C14.<br>3. Using the similar method, the incorporation of the labeled carbon of hypoxanthine-8-C14 or D-ribose-1-C14 in deoxyinosine was studied. In the presence of hypoxanthine-8-C14, D-ribose and CN-B12, the labeled carbon was found in the deoxyinosine fraction by paper chromatoscanning in the amount of 2.7% of the added C14, but in the presence of hypoxanthine, D-ribose-1-C14 and CN-B12, the incorporation of the labeled carbon into deoxyinosine was scarcely detected.

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