A simple method to survey a ratio of wheat and barley seeds infested by <i>Pseudomonas syringae</i> pv. <i>syringae</i> using multi-well plate

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  • マルチウェルプレートを用いたムギ種子の簡便な黒節病菌保菌粒率調査法
  • マルチウェルプレート オ モチイタ ムギ シュシ ノ カンベン ナ クロフシビョウキンホキンリュウリツ チョウサホウ

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Abstract

A simple method to survey a ratio of barley or wheat seeds infested by Pseudomonas syringae pv. syringae (Pss). Barley or wheat seeds were put in multi-well plate and immersed in 200 μl sterile water at 8°C for 3 days. A small aliquot of the seed immersed water was transferred on selective medium to detect Pss using 48-pin copy plate, and incubated at 25°C for 7 days. Black colonies of Pss were clearly detected without contamination of filamentous fungi. This is a simple and easy method to examine many seeds at a time, and it is expected to detect infested seeds more precisely.

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