Selective Absorption of Dietary Sphingoid Bases from the Intestine via Efflux by P-Glycoprotein in Rats
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- FUJII Aoi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- MANABE Yuki
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- AIDA Kazuhiko
- Innovation Center, Nippon Flour Mills Co., Ltd.
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- TSUDUKI Tsuyoshi
- Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University
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- HIRATA Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University Department of Rehabilitation, Shijonawategakuen University
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- SUGAWARA Tatsuya
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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抄録
Various physiological functions of dietary sphingolipids, such as preventing inflammation and improving the skin barrier function, have been recently demonstrated. The sphingolipid most commonly used as a foodstuff is glucosylceramide from plant sources, which is composed of sphingoid bases that are distinctive from those found in mammals. Although the structure of sphingoid bases in higher plants is more complicated than the structure of those in mammals, the fate of dietary sphingolipids of plant origin is still not understood. In the present study, we investigated the absorption of 4,8-sphingadienine that originated from maize glucosylceramide in the rat intestine by using a lipid absorption assay of lymph collected from the thoracic duct. The cumulative recovery of 4,8-sphingadienine in the lymph was lower than that of sphingosine. Verapamil, a P-glycoprotein inhibitor, significantly increased the absorption of 4,8-sphingadienine but did not affect the absorption of sphingosine. Plant-derived sphingoid bases were detected in the ceramide fraction of lymph fluid by using liquid chromatography-mass spectrometry analysis. These results indicate that 4,8-sphingadienine that originates from the glucosylceramide of higher plants is poorly absorbed in the intestine because of efflux by P-glycoprotein and can be incorporated into a ceramide moiety, at least in part, in intestinal endothelial cells.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 63 (1), 44-50, 2017
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詳細情報 詳細情報について
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- CRID
- 1390001206324767616
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- NII論文ID
- 130005492529
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- NII書誌ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 028009232
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- PubMed
- 28367925
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可