Higher Concentrations of Interferon-γ Enhances Uptake and Transport of Dietary Antigens by Human Intestinal Cells: A Study Using Cultured Caco-2 Cells

DOI Web Site 参考文献36件
  • KODA Tomoko
    Department of Nutrition. Faculty of Human Life Sciences, Yamaguchi Prefectural University
  • MINAMI Hisanori
    Division of Food and Health Environment, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
  • OGAWA Tadashi
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
  • YAMANO Mariko
    Department of Rehabilitation, Osaka Prefecture College of Nursing
  • TAKEDA Eiji
    Department of Clinical Nutrition, School of Medicine, Tokushima University

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タイトル別名
  • Higher Concentrations of Interferon-.GAMMA. Enhances Uptake and Transport of Dietary Antigens by Human Intestinal Cells: A Study Using Cultured Caco-2 Cells
  • Higher Concentrations of Interferon ガンマ Enhances Uptake and Transport of Dietary Antigens by Human Intestinal Cells A Study Using Cultured Caco 2 Cells

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Besides functioning as a mucosal barrier and transporting nutrients, intestinal epithelial cells (IECs) also serve as antigen-presenting cells (APCs). Modification of protein antigens by proteolysis is one of the principal steps in antigen presentation to Th cells. We used a Caco-2 intestinal epithelial cell line to investigate the transepithelial transport of the dietary antigen, ovalbumin (OVA). We also examined the effects of the proinflammatory cytokine interferon-γ (IFN-γ) on the antigen transport process in Caco-2 cell layers. Caco-2 cell layers transferred both intact and degraded OVA from the mucosal to the serosal side. IFN-γ stimulated OVA transport and most of the transported OVA in such cells were degraded. We also examined OVA uptake by Caco-2 cells using immunohistochemical means. Caco-2 cells incorporated OVA in a time-dependent manner and IFN-γ significantly enhanced antigen internalization. Flow cytometry also demonstrated that IFN-γ elevated the internalization of FITC-OVA. We also determined the effects of low and high concentrations of IFN-y on mucosal permeability and internalization of FITC-OVA. Althoughh both 10 and 50ng/mL IFN-γ stimulated mucosal permeability to the same extent, more FITC-OVA was internalized by Caco-2 cells incubated with 50 than with 10ng/mL IFN-y. These results suggest that the effects of IFN-γ on mucosal permeability and the internalization of antigens by intestinal epithelial cells are brought about by different mechanisms. Therefore, higher concentrations of IFN-γ stimulate the uptake, processing, and transport of dietary antigens by IECs.

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