Some characteristics of cytosol binding protein for 1.ALPHA.,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in rat intestinal mucosa.
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- SHIMURA Fumio
- Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
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- MORIUCHI Sachiko
- Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
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- HOSOYA Norimasa
- Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
Bibliographic Information
- Other Title
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- Some Characteristics of Cytosol Binding Protein for 1α,25-Dihydroxycholecal-ciferol and 25-Hydroxycholecalciferol in Rat intestinal Mucosa
- Some Characteristics of Cytosol Binding
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Abstract
Cytosol binding protein for 1α, 25-(OH)2D3 and 25-OHD3 was demonstrated in rat intestinal mucosa using sucrose density gradient ultracentrifugation analysis, Sephadex G-200 column chromatography and polyacrylamide disc gel electrophoresis. The binding protein has a sedimentation constant of 5-6S, a molecular weight of 100, 000-120, 000 daltons and a mobility in electrophoresis of Rf 0.42. Further analysis of binding behavior by DEAE-cellulose filter assays revealed that the binding protein had a higher affinity for 25-OHD3 than for 1α, 25-(OH)2D3, and that 1α, 25-(OH)2D3 competed with 25-OHD3 for the same binding site on the protein. The apparent dissociation constant for 1α, 25-(OH)2D3 and 25-OHD3 was 9.2×10-9 M and 1.5×10-9 M, respectively. The binding capacity was 3.1 pmoles/mg protein for both 1α, 25-(OH)2D3 and 25-OHD3. The order of binding affinity was 25-OHD3>1α, 25-(OH)2D3>>D3=1α-OHD3
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 23 (3), 187-200, 1977
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206325239424
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- NII Article ID
- 130001372307
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- NII Book ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 1828609
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- PubMed
- 915553
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed