The isolation and characterization of a lethal protein from kintoki beans (Phaseolus vulgaris).
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- 浜口 陽一
- Department of Food Science and Nutrition, Nara Women's University
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- 八木 典子
- Department of Food Science and Nutrition, Nara Women's University
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- 西野 明子
- Department of Food Science and Nutrition, Nara Women's University
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- 望月 てる代
- Department of Food Science and Nutrition, Nara Women's University
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- 水上 戴子
- Department of Food Science and Nutrition, Nara Women's University
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- 三好 正満
- Department of Food Science and Nutrition, Nara Women's University
書誌事項
- タイトル別名
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- Isolation and Characterization of a Let
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抄録
A lethal protein with hemagglutinating activity but with-out trypsin inhibitory activity was isolated from beans of Phaseolus vulgaris, cultiva, and Kintoki and proved homogeneous by ultracentri-fugation, disc polyacrylamide gel electrophoresis, sodium dodesyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. The mole cular weight was estimated to be 104, 000 by ultracentrifugal analysis and gel filtration on Sephadex G-200. The molecule dissociates into three identical subunits in the presence of 8M urea or 0.1% sodium dodesyl sulfate. The amino acid composition was characterized by the high content of aspartic acid and the complete absence of methionine and cystine. The carbohydrate content was 8.1%; 5.0% mannose and 3.1% glucosamine. The addition of the lethal protein to a basal diet (0.4%) resulted in the intensive depression of the growth and finally in the death of rats. The intraperitoneal injection of 250μg per g body weight of mouse brought about an acute toxicity which caused death of all the injected mice.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 23 (6), 525-534, 1977
一般財団法人 学会誌刊行センター
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詳細情報 詳細情報について
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- CRID
- 1390001206325379456
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- NII論文ID
- 130001372265
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- NII書誌ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 1916900
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- PubMed
- 611161
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可