Isolation and purification of in vivo-generated 25-hydroxyvitamin D2 by preparative high-performance liquid chromatography.
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- 岡野 登志夫
- Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
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- 松山 直子
- Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
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- 小林 正
- Department of Hygienic Chemistry, Kobe Women's College of Pharmacy
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- 黒田 英造
- Department of Pediatrics, Kobe University School of Medicine
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- 児玉 荘一
- Department of Pediatrics, Kobe University School of Medicine
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- 松尾 保
- Department of Pediatrics, Kobe University School of Medicine
書誌事項
- タイトル別名
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- Isolation and Purification of In Vivo G
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In order to obtain a standard compound of 25-hy-droxyvitamin D2 (25-OH-D2), a method for isolating in vivo-generated 25-OH-D2 from the blood of rats or rabbits was established by using several steps of preparative high-performance liquid chromatography (HPLC). When the unsaponifiable matter of the plasma obtained from rats or rabbits receiving a large dose of vitamin D2 was applied to the preparative HPLC using a Zorbax SIL column, a peak denoted as peak X was observed on the chromatogram. Since the peak X was thought to be due to 25-OH-D2 from the experiments of time course and dose-response, it was purified by subjecting it to successive preparative HPLC using several kinds of columns. From the results of ultraviolet (UV) absorption spectrum, gas chromatography-mass spectrometry (GC-MS) and mass chromatography, the purified peak X compound was confirmed to be 25-OH-D2. The proposed method for isolating in vivo-generated 25-OH-D2 is very convenient, because the time to perform each HPLC is very short though several steps of HPLC are used.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 25 (6), 479-493, 1979
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詳細情報 詳細情報について
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- CRID
- 1390001206325409664
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- NII論文ID
- 130001371977
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- NII書誌ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 2204471
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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