Time Reduction of Vitamin B₆ and Inositol Assay by Using Lyophilized Saccharomyces cerevisiae ATCC 9080
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- TSUDA Harutoshi
- Department of Food Function and Labeling, National Institute of Health and Nutrition
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- MATSUMOTO Teruki
- Department of Food Function and Labeling, National Institute of Health and Nutrition
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- ISHIMI Yoshiko
- Department of Food Function and Labeling, National Institute of Health and Nutrition
書誌事項
- タイトル別名
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- Time Reduction of Vitamin B<sub>6</sub> and Inositol Assay by Using Lyophilized <i>Saccharomyces cerevisiae</i> ATCC 9080
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抄録
The purpose of this study was to improve the efficiency of the microbiological assays for vitamin B6 and inositol by using lyophilized cells from Saccharomyces cerevisiae ATCC 9080. The use of lyophilized cells as an inoculum was assessed to avoid time-consuming processes like cell precultivation and washing. The authors also examined the effects of various protectants such as skim milk, lactose, maltose, and trehalose on the assay. The viable cell counts of the lyophilized cells were approximately equal. The standard curves for vitamin B6 and inositol concentrations obtained using lyophilized cells with maltose and intact cells gave similar linear ranges. Furthermore, the measured vitamin concentrations of the standard reference material 1849 were in the range of the established values. Therefore, lyophilized cells with maltose are potential alternative inocula for the turbidimetric method. This will increase the overall convenience associated with microbiological assays.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 58 (2), 149-151, 2012
一般財団法人 学会誌刊行センター
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詳細情報 詳細情報について
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- CRID
- 1390001206325718272
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- NII論文ID
- 130002103033
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BC38XmsFymtLo%3D
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 023599775
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- PubMed
- 22790574
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可