Fluorescence Dipolarization Studies of Phase Transition and Fluidity in Lecithin Liposomes Containing α-Tocopherol
書誌事項
- タイトル別名
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- Fluorescence depolarization studies of phase transition and fluidity in lecithin liposomes containing .ALPHA.-tocopherol.
- Fluorescence Dipolarization Studies of
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説明
The effects of α-tocopherol and related compounds on the membrane fluidity of liposomes composed of dipalmitoyl-lecithin or egg lecithin were studied by measuring the fluorescence polarization of DPH (1, 6-diphenyl-1, 3, 5-hexatriene), a hydrophobic fluorescent probe distributed in the hydrocarbon region of lipid bilayers. The transition temperature of dipalmitoyl-lecithin liposomes was lowered on incorporation of α-tocopherol into the membranes. A similar effect was observed on incorporation of phytol or phytanic acid into liposomes, but not on incorporation of 2, 2, 5, 7, 8-pentamethyl-l-hydroxychroman as an α-tocopherol model compound (TMC) which lacks an isoprenoid side chain. Above the phase-transition temperatures, incorporation of α-tocopherol or TMC dose-dependently increased the polarization values of DPH in dipalmitoyl-lecithin and egg lecithin liposomes, while incorporation of phytol or phytanic acid had little effect or slightly lowered the polarization. These results suggest that the effect of α-tocopherol in lowering the phase-transition temperature depends on its hydrophobic side chain, and that its effect in decreasing the membrane fluidity above the phase-transition temperature depends on its hydrophylic chroman ring portion. Cholesterol had an effect similar to α-tocopherol on membrane fluidity, αTocopherol and cholesterol had independent effects in increasing the polarization of DPH above the phase-transition temperature when the two compounds were incorporated together into liposomes.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 26 (5), 427-434, 1980
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詳細情報 詳細情報について
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- CRID
- 1390001206325882240
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- NII論文ID
- 130001371841
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DyaL3MXis1Ggtg%3D%3D
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 2337149
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- PubMed
- 6894310
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- NDLサーチ
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- PubMed
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- 使用不可