Semi-automated system for analysis of vitamin B6 complex by ion-exchange columin chromatography.
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- YASUMOTO Kyoden
- Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
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- TADERA Kenjiro
- Department of Food Science & Technology, Faculty of Agriculture, Kyoto University Department of Agricultural Chemistry, Faculty of Agriculture, Kagoishima Univeristy
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- TSUJI Hideaki
- Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
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- MITSUDA Hisatern
- Department of Food Science & Technology, Faculty of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- Semi-automated system for analysis of vitamin B6 complex by ion-exchange column chromatography
- Semi automated system for analysis of v
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Abstract
A simple system for semi-automatic analysis of pyridoxal, pyridoxine and pyridoxamine has been developed using diazide of 5-chloroaniline 2, 4-disulfonyl chloride as a color-producing reagent in combination with desoxypyridoxine as an internal standard. It employs Aminex A-5 column, 10×0.9∅cm, as an adsorbent. Adsorbates were eluted successively with 3 discrete phosphate buffers (0.4N Nat). The effluent is mixed continuously with capillary streams of the diazide reagent and of sodium acetate. The mixture, maintained at 65°C in a heating bath, then passes through a spiral of Teflon tubing with a residence time of 2min. Characteristic orange colored products formed by a diazo coupling reaction are continuously monitored at 440nm in a flow photometer. The individual peaks on the recorded chromatogram are manually integrated by a conventional HW method. The elution position and recovery of desoxypyridoxine permits correction for sensitivity changes or mechanical losses which might occur during a series of analyses. The analyzer system described allow quantitation from 2 to 25μg of pyridoxal, pyridoxine and pyridoxamine in a single sample within 2 hr and with a precision of 100±4%. It is also found suitable as a routine procedure for the analysis of varied biological samples.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 21 (2), 117-127, 1975
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206326159104
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- NII Article ID
- 130001372708
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- NII Book ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 1596175
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- PubMed
- 1151502
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed