-
- OHURA Toshihiro
- Department of Pediatrics, School of Medicine, Tohoku University
-
- KOMINAMI Eiki
- Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, The University of Tokushima
-
- KATUNUMA Nobuhiko
- Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, The University of Tokushima
Bibliographic Information
- Other Title
-
- New Sensitive and Convenient Assay of O
Search this article
Abstract
A new radioisotopic assay for ornithine aminotransferase with [U-14C]ornithine as a substrate was developed. The dihydroquinazolinium compound formed was extracted from the reaction mixture with n-butanol and an addition of high concentration of sodium sulfate. This assay is sensitive and convenient and more than 50 assays can be done in one day without any special apparatus. The activities of ornithine aminotransferase in rat liver and human lymphoblastoid cells could be measured at nanomole levels by this method. Thus, the assay should be useful for measurement of OAT activity in cultured cells and for studies on the metabolic bases of the different types of ornithine aminotransferase deficiency: vitamin B6-responsive and -nonresponsive types
Journal
-
- Journal of Nutritional Science and Vitaminology
-
Journal of Nutritional Science and Vitaminology 29 (2), 123-128, 1983
Center for Academic Publications Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206326247168
-
- NII Article ID
- 130001371433
-
- NII Book ID
- AA00703822
-
- ISSN
- 18817742
- 03014800
-
- NDL BIB ID
- 2627856
-
- PubMed
- 6350545
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed