A stepwise detection system of obligate parasitic fungi in soils, <i>Olpidium bornovanus</i> that is the vector of <i>melon necrotic spot virus </i>

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  • メロンえそ斑点ウイルスを媒介する<i>Olpidium bornovanus </i>土壌汚染程度の段階的検出法
  • メロンえそ斑点ウイルスを媒介するOlpidium bornovanus土壌汚染程度の段階的検出法
  • メロンエソハンテン ウイルス オ バイカイ スル Olpidium bornovanus ドジョウ オセン テイド ノ ダンカイテキ ケンシュツホウ
  • A stepwise detection system of obligate parasitic fungi in soils, Olpidium bornovanus that is the vector of melon necrotic spot virus

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Abstract

A few Olpidium species are recognized as fungal vectors of plant viruses. For the control of viral diseases caused by Olpidium species, it is important to quantitatively detect the presence of such fungi in field soil. We developed a quantitative detection system for Olpidium bornovanus being able to transmit Melon necrotic spot virus. We constructed a direct direction assay that detected fungal-specific bands from DNA isolated from soil using PCR analysis, and an indirect detection assay that combined fungal propagation in the root of the host plant and detection of specific bands from root DNA by PCR analysis. The results of these detection assays indicated that detectable sensitivity of the indirect detection assay was higher than that of the direct detection assay. We believe that the indirect detection assay is practical and useful for the quantitative detection of the pathogenic fungus.

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