Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis.
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- HARAYAMA Hiroshi
- Division of Bioscience, Graduate School of Science and Technology , Kobe University, Kobe 657, Japan
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- MIYANO Takashi
- Department of Animal Science, Faculty of Agriculture, Kobe University, Kobe 657, Japan
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- MASUDA Hiroshi
- Department of Animal Reproduction, National Institute of Animal Industry, Tsukuba 305, Japan
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- MIYAME Masashi
- Division of Bioscience, Graduate School of Science and Technology , Kobe University, Kobe 657, Japan
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- KATO Seishiro
- Department of Animal Science, Faculty of Agriculture, Kobe University, Kobe 657, Japan
書誌事項
- タイトル別名
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- Detection of 25 kDa Anti Agglutinin in
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Distribution of the 25-kDa epididymal anti-agglutinin for spermatozoa was examined by the Western blotting technique in fluid collected from boar rete testes and epididymides. Fluid was collected from the rete testes and various regions of the epididymis, and then plasma and sperm extracts were prepared. Antiserum was raised against the anti-agglutinin, and specific antibody was purified from it. In the plasma, a reaction between the anti-agglutinin and purified antibody was first observed in the proximal corpus epididymidis and became stronger in the more distal regions. In extracts from washed spermatozoa using the original sample buffer, anti-agglutinin was detected between the proximal corpus and the distal cauda and more strongly in the distal corpus. A thorough washing of spermatozoa on a discontinuous Percoll gradient brought about only minor changes in the detection pattern of anti-agglutinin. However, in samples extracted from washed spermatozoa using a modified sample buffer containing twofold detergent and twofold 2-mercaptoethanol, a stronger reaction was observed between the anti-agglutinin and purified antibody in the distal cauda than in the distal corpus. These results indicate that free or loosely sperm-bound anti-agglutinin increases in the corpus and cauda epididymides. It is also suggested that solubility of the anti-agglutinin associated with spermatozoa in the original sample buffer decreases in the cauda epididymidis.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 41 (2), 113-121, 1995
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206332309632
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- NII論文ID
- 10027316774
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK2MXovVajtLk%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 3644125
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可