Stage-Dependent Viability of Mouse Preimplantation Embryos Vitrified with Sugar-containing Solutions.
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- TADA Norihiro
- Laboratory for Pathobiology
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- SATO Masahiro
- Laboratory for Pathobiology
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- AMANN Egon
- Molecular Biology, Pharma Research Laboratories, Hoechst Japan Ltd.
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- OGAWA Shyoso
- Laboratory for Reproduction Engineering, Department of Animal Production, Meiji University
書誌事項
- タイトル別名
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- Stage Dependent Viability of Mouse Prei
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The aim of the work is to determine the susceptibility of mouse preimplantation embryos to vitrification at different developmental stages. The experiment was carried out in embryos at 1-cell, morula and blastocyst stages. As a vitrification solution, a mixture of 2.5 M dimethylsulphoxide + 2.5 M propylene glycol (DP), DP + 1.0 M sucrose (DPS) or DP + 0.16 M raffinose (DPR), was used. The in vitro survival, evaluated by the development into a late blastocyst stage in vitro, of control and vitrified mouse embryos was examined. A high survival rate (80%) after vitrification was obtained for 1-cell embryos after a 15 sec-exposure to DPS. High survival rates for morulae (91-96%) and relatively low survival rates for blastocysts (30-55%) were observed after vitrification in either DPS or DPR after short exposures (within 30 sec). In vivo survival rate for each embryos vitrified in DPS by a short exposure (within 30 sec) at 1-cell, morula or blastocyst stage was 56, 53 or 40%. Though the rates for 1-cell and molura stages were comparable with those in control embryos (55 and 58%), the rate for blastocyst stage was significantly lower (P<0.05) than that in control embryos (56%). Thus, the proposed vitrification procedure can be useful in the cryopreservation of mouse preimplantation embryos at 1-cell and morula stages.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 39 (2), 139-144, 1993
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206332532608
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- NII論文ID
- 130000845996
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK3sXltlKhsrg%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 3828096
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可