A double-antibody enzyme immunoassay for determination of gonadotropin-releasing hormone in bovine plasma.
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- GESHI Masaya
- Department of Animal Reproduction, College of Agriculture, University of Osaka Prefecture Tohoku National Agricultural Experiment Station
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- MATSUDA Kenji
- Department of Animal Reproduction, College of Agriculture, University of Osaka Prefecture
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- MORI Junichi
- Department of Animal Reproduction, College of Agriculture, University of Osaka Prefecture
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- KASUGA Naoki
- Research Division, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
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- IMAGAWA Kenichi
- Research Division, Otsuka Assay Laboratory, Otsuka Pharmaceutical Co., Ltd.
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- YANAIHARA Noboru
- University of Shizuoka, School of Pharmaceutical Sciences
Bibliographic Information
- Other Title
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- 牛における性腺刺激ホルモン放出ホルモンの二抗体法enzyme immunoassay
- ウシ ニ オケル セイセン シゲキ ホルモン ホウシュツ ホルモン ノ 2 コ
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Abstract
A double-antibody enzyme immunoassay (EIA) for the determining GnRH in bovine plasma was studied. Antiserum raised against [Glu1]-GnRH-rabbit serum albumin conjugate in a male horse was used. Enzyme-labelled antigens were prepared by coupling synthetic GnRH with horseradish peroxidase using bis-diazotized benzidine. GnRH analogues and fragment of GnRH were tested for cross-reactivity in the EIA system. These results suggested that the C-terminus of GnRH molecule plays more important role in antigen-antibody binding than the N-terminus of it.<BR>Plasma specimens were extracted with acid-methanol. Process standard curve was suitable for plasma assay. The sensitivity of this EIA system was as little as 50 pg/tube of GnRH. After intravenous injection of 200 μg of synthetic GnRH in 2 steers, plasma concentrations of GnRH were determined in this EIA system. The levels abruptly increased just after injection and decreased thereafter.
Journal
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- The Japanese journal of animal reproduction
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The Japanese journal of animal reproduction 34 (4), 243-248, 1988
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
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Details 詳細情報について
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- CRID
- 1390001206332725632
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- NII Article ID
- 130004214181
- 40004157416
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- NII Book ID
- AN00041843
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- NDL BIB ID
- 3224773
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- ISSN
- 03859932
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed