Fertilizing ability of mouse oocytes matured in vitro.

DOI Web Site Web Site
  • MINATO Yoshiaki
    School of Veterinary Medicine and Animal Sciences, Kita-sato University A.I. Research Section, Livestock Impro-vement Technical Center, Livestock Improvement Association of Japan
  • TOYODA Yutaka
    School of Veterinary Medicine and Animal Sciences, Kita-sato University

Bibliographic Information

Other Title
  • 体外成熟マウス卵子の受精能力について
  • タイガイ セイジュク マウス ランシ ノ ジュセイ ノウリョク ニ ツイテ

Search this article

Abstract

Primary oocytes surrounded with cumulus cells were obtained from ovarian follicles of prepuberal mice of JCL: ICR strain (25-27 days old) which had been injected with 5 IU PMSG 46-47h previously. The oocytes were cultured for 12h to induce meiotic maturation and then inseminated in vitro with epididymal spermatozoa.<BR>Fertilization was assessed 11-15h later. When the oocytes were matured and fertilized in the basic medium which is a chemically defined medium for in vitro fertilization of mouse oocytes, 38.4% (109/284) of the oocytes showed the evidence of sperm penetration into the vitellus but only 28.4% of these oocytes were found to have male and female pronucleus. The other penetrated oocytes showed either fertilizing sperm tail(s) only or incompletely enlarged sperm head(s) with chromosomes ar-rested or degenerating at metaphase II.<BR>Addition of fetal calf serum (FCS, 1-20%) to the basic medium resulted in an increase in the number of oocytes fertilized (82.1-92.7%) and of the fertilized oocytes with male and female pro-nucleus (59.8-89.5%). Non-dialysable fraction of FCS (5-20%) was effective in increasing the fertiliza-tion rates (53.8-69.0%), while the ultrafiltrated fraction of FCS (5%, molecular weight<10, 000) was effective in increasing the proportion of fertilized oocytes that had male and female pronucleus (72.0%).

Journal

Details 詳細情報について

Report a problem

Back to top