Effect of Brefeldin-A on the Development of Mouse 4-Cell Embryos In Vitro.
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- OGAWA Hidehiko
- Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
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- MORI Tadashi
- Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
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- SHIMIZU Hiroshi
- Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
書誌事項
- タイトル別名
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- Effect of Brefeldin-A on the Developmen
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抄録
The effect of the protein secretion inhibitor, brefeldin-A on the development of mouse 4-cell embryos was investigated. Four-cell embryos of various ages (0, 2, 3, 4, 5, 6, 7, and 8 h post division to the 4-cell stage) were cultured continuously in a medium containing 5 μg/ml brefeldin-A, and the embryos were observed for the evidence of cell division. Developmental rates to the 8-cell stage were significantly lower in embryos cultured with brefeldin-A from 0, 2, 3, 4 and 5 h post division to the 4-cell stage (16.4%, 25.0%, 42.9%, 56.7% and 56.9%, respectively). The embryos placed in BFA from 6, 7 and 8 h post division to the 4-cell stage cleaved normally (85.5%, 94.0% and 100%, respectively). Some of the embryos in which cell division did not occur consisted of 2 or 3 blastomeres that were binucleate. However, when the brefeldin-A application was limited to 6 h, the ability of cell division was reverted (more than 80%). Most of these embryos formed blastocoel cavity by 40 h post division to the 4-cell stage as normal embryos.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 43 (2), 151-156, 1997
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206333528832
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- NII論文ID
- 130000055240
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK2sXltlWkurg%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 4240398
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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