The bitter peptide in Natto.

DOI

Bibliographic Information

Other Title
  • 納豆中の苦味ペプチド

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Description

The extract from Natto with n-butanol was separated into bitter peptide-rich fractions and some other fractions by Sephadex G-25 gel-filtration and ion-exchange resin chromatography on CM-Sephadex. The main peptide component was subjected to further purification by high voltage paper electrophoresis. The major spot of peptide on the electropherogram was cut off, and eluted with 10% acetic acid. The amino acid composition of purified peptide was Asp 1, Thr 1, Glu 1, Ala 1, Pro 2, Val 3, Ile 3 and Leu 5. amino acid sequence of the purified peptide was determined by DNP method for N-terminal and Carboxypeptidase A method for C-terminal sequence. The N-terminal amino acid of the peptide was found to be Leu and the C-terminal sequence of the peptide was -Ala-Val-Ile-Leu.

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Details 詳細情報について

  • CRID
    1390001206333585920
  • NII Article ID
    130003850688
  • DOI
    10.11428/jhej1987.38.93
  • COI
    1:CAS:528:DyaL2sXktlygsrY%3D
  • ISSN
    18820352
    09135227
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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