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The bitter peptide in Natto.
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- YAMASAKI Yoshio
- Sakuyo Junior College
Bibliographic Information
- Other Title
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- 納豆中の苦味ペプチド
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Description
The extract from Natto with n-butanol was separated into bitter peptide-rich fractions and some other fractions by Sephadex G-25 gel-filtration and ion-exchange resin chromatography on CM-Sephadex. The main peptide component was subjected to further purification by high voltage paper electrophoresis. The major spot of peptide on the electropherogram was cut off, and eluted with 10% acetic acid. The amino acid composition of purified peptide was Asp 1, Thr 1, Glu 1, Ala 1, Pro 2, Val 3, Ile 3 and Leu 5. amino acid sequence of the purified peptide was determined by DNP method for N-terminal and Carboxypeptidase A method for C-terminal sequence. The N-terminal amino acid of the peptide was found to be Leu and the C-terminal sequence of the peptide was -Ala-Val-Ile-Leu.
Journal
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- Journal of Home Economics of Japan
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Journal of Home Economics of Japan 38 (2), 93-97, 1987
The Japan Society of Home Economics
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Details 詳細情報について
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- CRID
- 1390001206333585920
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- NII Article ID
- 130003850688
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- COI
- 1:CAS:528:DyaL2sXktlygsrY%3D
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- ISSN
- 18820352
- 09135227
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed