走査型電子顕微鏡による牛の生殖道粘膜上皮の観察

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  • 林 孝
    農林水産省東北農業試験場畜産部
  • 松川 正
    農林水産省東北農業試験場畜産部

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タイトル別名
  • Scanning electron microscopical study on epithe-lia of the bovine genital tract
  • ソウデンシ ケンビキョウ ニヨル ウシ ノ セイショク ドウネンマク ジョウヒ

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Genital tract mucosa obtained from eleven cows at various reproductive phase were examined by scanning electron microscopy.<BR>Cows were consisted of Holstein and Japanese black. The specimens dissected from uterus, cervix and vagina were fixed with glutaraldehyde rinsed with phosphate buffer, dehydrated with graded series of alcohol, then transfered from alcohol into isoamylacetate. Air drying and critical point drying from isoamylacetate were applied. Thereafter, dried tissues were coated with carbon and gold, and were subjected to examination with JEOL JSM-35.<BR>During follicular phase, ciliated cells were less abundant in the endometrial surface than in the oviductal epithelium, and the surface of non-ciliated cell was coated with thin mucus. Hexagonal cells and secretion-like structures composed with disc-like units were observed in the endometrium during luteal phase. The cells coated with spares microvilli were located in the endometrial surface at 60 days of pregnancy. In estrus, cervical epithelium was coated with thick mucus, and string-like structures were identified in the mucus. At luteal phase, the cervical epithelium contained ciliated cells and non-ciliated cells with smooth surface or rough surface. In vaginal mucosa, the cells coated with thin mucus and the spongy cells possibly corresponding to degeneration were observed at follicular phase. Squamous stratified epithelium covered the surface of vaginal mucosa at luteal phase.<BR>The surface topology of these genital epithelia was illustrated by photographs and discussed.

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