書誌事項
- タイトル別名
-
- An attempt of the development of solid-phase enzyme immunoassay of gonadotropin releasing hormone.
- セイセン シゲキ ホルモン ホウシュツ ホルモン ノ コソウホウ enzyme
この論文をさがす
抄録
A solid-phase competitive enzyme immunoassay of gonadotropin releasing hormone (GnRH) was examined. Enzyme-labeled antigens were prepared by coupling synthetic GnRH with peroxidase using bis-diazotized benzidine and purified by Sephadex G-25 column chromatography. Antiserum raised against [Glu1]-GnRH-rabbit serum albumin conjugate in a male horse was used. Immunoglobulin G (IgG) fraction of the antiserum used for a solid-phase was prepared by an (NH4)2SO4 precipitation method, purified by Sephacryl S-300 column chromatography, and adsorbed to a polystyrene ball.<BR>Three-tenths ml of 0.05 M phosphate buffer (pH 7.5), 0.1 ml of standard or sample solution, 0.1 ml of peroxidase-labeled GnRH solution and a solid-phase polystyrene ball were added in a test tube. After incubation at 4°C for 24 hours, a polystyrene ball was washed three times with 2 ml of saline, and transferred to a new tube. The enzyme activity was measured with a spectrophotometer (absorbance at 492 nm).<BR>A dose-response curve was obtained between 1 to 1000 ng/ml of GnRH when a solid-phase coated with 50 μg/ml IgG solution and peroxidase-labeled GnRH solution (×100) were used. Acid extract of bovine pituitary stalk produced dose-response curve indistinguishable from synthetic GnRH, but those of anterior lobe, liver and muscle didn't produced.<BR>These results show that GnRH is assayed by this method.
収録刊行物
-
- 家畜繁殖学雑誌
-
家畜繁殖学雑誌 32 (4), 172-176, 1986
日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206333689216
-
- NII論文ID
- 130004214131
- 40004157299
-
- NII書誌ID
- AN00041843
-
- NDL書誌ID
- 3122579
-
- ISSN
- 03859932
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可