Cultivation in vitro of bovine embryos in a medium supplemented with bovine serum collected 7-8 days after superovulation.

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  • 発情後７～８日目に採取した供卵牛血清加培養液によるウシ受精卵の体外培養
  • ハツジョウゴ 7 8ニチメ ニ サイシュシタ キョウラン ウシ ケッセイ カバ

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Cultivation in vitlo of bovine embryos in a medium supplemented with bovine serum which was collected from 23 superovulated cows 7 to 8 days after estrus.<BR>Bovine embryos at the stages of morula and blastocyst were cultured at 38 C for 72 h in a stoppered test tube containing 1 ml medium supplemented with 20% bovine serum.<BR>When embryos were cultured in a modified PBS with serum collected on day 7-8 from cows which produced good embryos, 49/57 embryos (86%) were developed to the blastocyst or hatched blastocyst and 83% of the embryos were finally hatched after 72 h of cultivation. On the other hand, when serum, collected from donor cows which produced poor embryos, were supplemented to the medium, none of the 24 embryos was developed.<BR>Effect of bovine serum collected from donor cows on day 7-8 on the developement in vitro of bovine embryo was compared with that of foetus calf serum (FCS). When 20% bovine serum was supplemented to the medium, all 17 frozen-thawed embryos were developed to the blastocyst or hatched blastocyst and the proportion of hatched blastocysts was finally 70%. In contrast, when 20% FCS was supplemented to the medium, 7 of 12 frozenthawed embryos 58% were developed to the blastocystor hatched blastocyst and the final proportion of the hatched blastocysts (22%)was also lower than the rate obtained in the medium including bovine serum.

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