誘起排卵マウス（ＪＣＬ‐ＩＣＲ）における胎児体重と胎児数の関係

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  • Relationship between fetal body weight and number of fetuses in JCL-ICR mice following gonadotropin treatment
  • ユウキ ハイラン マウス JCL ICR ニ オケル タイジ タイジュウ ト

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Weights of fetuses in JCL-ICR mice were studied in relation to the litter size and denaturating fetuses. Virgin females of mice were injeced with 5 IU of PMSG, followed 48 hours later by 5 IU of HCG, paired with males, and examined the following day (day 1 of gestation) for vaginal plugs. On day 14, 15, 16, 17, 18 and 19 of gestation, females were killed and the number of fetuses was recorded. Each live fetus in each horn was weighed to the nearest 0.01 g. One hundred and thirty eight of 314 mated mice beared live fetuses at the time of autopsy. Data derived from the 138 pregnancies were analysed. Out of a total of 1868 fetuses obtatned from 138 mice, 1806 were live fetuses, and 62 were denaturating; 844 live fetuses were in the left horn and 962 were in the right. The average number of live fetuses per mice was 13.0±4.9 (left: 6.1±2.6, right: 6.9±2.9) and the greatest number of live fetuses in pregnant mice was 32 and the smallest number was 2. The mean weight of live fetuses in mice having 812 live fetuses was greater than those in mice having 27 and 1432 fetuses. This suggesed the existence of the optimal number of fetuses for the fetal growth. The values calculated by the formula shown in table 1 were greater when the difference in the fetal number between the right and left horns was 5 on day 16, and 3 on day 19 of gesta-tion. The mean weight of live fetuses in mice having 3 or more denaturating fetuses was signifi-cantly smaller than that in mice having no denaturating fetuses.

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