Prenatal diagnosis of sex by the amniotic fluid incattle

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  • ウシの羊水による性別の出生前診断
  • ウシ ノ ヨウスイ ニヨル セイベツ ノ シュッショウゼン シンダン

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Abstract

For the purpose of prenatal diagnosis of sex in cattle, amniotic fluid was asepticaly collected, and the amniotic cells were cultured in vitro in 70 cases; 64 cases were obtained from the pregnant uterus at the time of slaughter, and 6 cases were collected from a pregnant cow.<BR>As the number of amniotic cells per 1ml of amniotic fluid was 5×103-105 (2-6 months of preg-nancy), 10-20ml of amniotic fluid was centrifuged at 1, 000rpm for 5 minutes, and the cell button was dispersed and suspended in a medium of 3ml. The cell cultures were prepared in an incubator at 37C from 8 to 37 days.<BR>Successful cultivation of amniotic cells was obtained by a medium supplemented with bovine fetal serum and N-2-Hydroxyethylpiperadine-N'-2-ethanesulfornic Acid rather than by the other pre-pared mediums.<BR>Amniotic cells from the 3 to 5 months of pregnancy attained the fullest growth in vitro as com-pared with those of the other months.<BR>Twelve cases of successful cultures from the pregnant uterus could be examined chromosomally, and the chromosomal sex and the gonadal sex of the fetus were in good agreement. In 5 out of 6 cases from the pregnant cows, the prenatal chromosomal sex and the gonadal sex of the calf after birth was in agreement, however, in 1 case, a discordance was observed between the prenatal chromo-somal sex and the neonatal gonadal sex.<BR>In addition, a rapid, simple dividing method between amniotic fluid and the allantoic fluid was designed to prevent the false aspiration of allantoic fluid in place of amniotic fluid.

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