Use of microtitre plate enzyme immunoassay kit for direct determination of progesterone in bovine blood plasma.

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  • HIRAKO Makoto
    Department of Animal Reproduction, National Institute of Animal Industry
  • KARIYA Takayosi
    Department of Animal Reproduction, National Institute of Animal Industry
  • DOMEKI Ikuo
    Department of Animal Reproduction, National Institute of Animal Industry

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Other Title
  • マイクロプレートを用いた酵素免疫測定法キットによる牛血しょう中プロゲステロンの直接測定

Abstract

A microtitre plate enzyme immunoassay (EIA) kit for the determination of the concentration of plasma progesterone was evaluated.<BR>The procedure of the EIA kit is very simple and brief; the assay completes in approximately 1.5 hours. Progesterone concentrations in bovine blood plasma can be measured without extraction.<BR>The intra-assay coefficients of variation (CV, N=10) of the absorbances and the concentrations were 4 to 5% and 8 to 16%, respectively. The inter-assay CV (N=8) were 9 to 15%. The inter-assay CV (N=6) of the absorbances of the provided standards in the same batch were 6 to 10%. The detection limit of progesterone was 2.5 pg/well.<BR>The correlation between progesterone concentrations in bovine blood plasma meas-ured by EIA and RIA was quite high (r=0.97, p<0.001).<BR>The results of the present examination suggest that the kit can be used to measure plasma progesterone with sufficient sensitivity and precision, and will be useful for laboratory and veterinary practices.

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