Antioxidative Compounds Contained in the Seed with Hard Shell of <i>Trapa Japonica</i> Flerov. and its Herbal Tea

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  • 菱の実及び菱茶に含有される抗酸化活性物質
  • ヒシ ノ ミノル オヨビ リョウチャ ニ ガンユウ サレル コウサンカ カッセイ ブッシツ

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Abstract

A hot water extract of herbal tea from the seed with hard shell of Trapa japonica Flerov. showed moderate antioxidative activity against the rat brain homogenate model. The antioxidative compounds in the hard shell were isolated by chromatographic methods, and identified for the first time as gallic acid (1), 1,2,3-trigalloyl-β-glucoside (2), 1,6-digalloyl-β-glucoside (3), 1,2,6-trigalloyl-β-glucoside (4), eugeniin (5), and 1, 2, 3, 6 tetragalloyl-β-glucoside (6) by using HR-MS and NMR analyses. Their respective antioxidative activities (IC50) were 14 μM (1), 5.2 μM (2), 8.8 μM (3), 0.25 μM (4), 0.26 μM (5), and 0.27 μM (6). The antioxidative compound in the herbal tea was also clarified as gallic acid (1) by an HPLC analysis.

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