ブタ胞はいのトロホブラストにおけるプロスタグランジンデヒドロゲナーゼの組織化学的研究

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タイトル別名
  • Histochemical studies of prostaglandin dehydro-genase in the trophoblast of pig blastocysts
  • ブタ ホウハイ ノ トロボブラスト ニ オケル prostaglandin d

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The activity of prostaglandin dehydrogenase in pig blastocysts at 13 days after mating was histochemically investigated by the NISSEN and ANDERSEN method with a slight modification, using prostaglandin (PG) A1, PGA2, PGB1., PGB2, PGE1, PGE2, PGF1a and PGF2a as the substrates, and using NAD and NADP as the cofactors. The composition of the medium was 0.3 mM substrate, 0.75 mM cofactor, 0.075mM nitro-BT and 0.1 M phosphate buffer solution at pH 8.0; the whole amount of the medium was 1 ml.<BR>The activity of NAD-dependent prostaglandin dehydrogenase was found in the trophoblast cells of the blastocyst when PGA1, PGB1, PGB2, PGE1 or PGE2 was used as the substrate, but it was not found with PGA2, PGF1a, or PGF2a. The activity of NADP-dependent enzyme was not demon-strated when either of all kinds of any PGs was used as the substrate.

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