マーモセット卵胞卵の体外培養

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  • 足達 雅之
    Laboratory of Primatology 財団法人実験動物中央研究所
  • 横山 峯介
    Animal Reproduction 財団法人実験動物中央研究所
  • 谷岡 功邦
    Animal Reproduction 財団法人実験動物中央研究所

書誌事項

タイトル別名
  • Culture of marmoset ovarian oocytes in vitro.
  • マーモセット卵胞卵の体外培養〔英文〕
  • マーモセット ランポウ ラン ノ タイガイ バイヨウ エイブン

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抄録

Oocytes recovered from or retained within the follicles dissected from ovaries of juvenile marmosets were cultured to examine the effect of follicular size, i.e. large (>800μm), medium (500-800μm) and small (<500μm), on the resumption and completion of meiotic maturation.<BR>The follicle-enclosed oocytes from all follicular groups failed to resume meiosis beyond the germinal vesicle stage, whereas the oocyte-cumulus cell complexes underwent the first meiotic division at a frequency which increased with the follicular growth. Maturation rates were 42.9% of oocytes from large, 22.2% from medium and 11.5% from small follicles. The incidence of degenerated oocytes decreased significantly as the fol-licular size increased.<BR>It is concluded that isolated oocytes from ovarian follicles of juvenile marmosets resume meiosis spontaneously in vitro and it was suggested that the ability to complete meiotic maturation is acquired during the stage of follicular growth.

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