Synergistic effects of glutathione and β-mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system supplemented with L-cysteine

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  • CHOE Changyong
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • SHIN Yong-Won
    Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine
  • KIM Eun-Jin
    Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine
  • CHO Sang-Rae
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • KIM Hyun-Jong
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • CHOI Sun-Ho
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • HAN Man-Hye
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • HAN Jaehee
    Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine
  • SON Dong-Soo
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA
  • KANG Dawon
    Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine

書誌事項

タイトル別名
  • Synergistic Effects of Glutathione and .BETA.-Mercaptoethanol Treatment During In Vitro Maturation of Porcine Oocytes on Early Embryonic Development in a Culture System Supplemented with L-cysteine
  • Synergistic effects of glutathione and v mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system supplemented with L cysteine

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Various methods have been used to remove reactive oxygen species (ROS) generated from in vitro culture (IVC) conditions that can cause cell injury or death, including the application of low oxygen (O2) tension and the addition of antioxidants. The beneficial effects of antioxidants and O2 tension on IVC of porcine embryos, however, are controversial among researchers. In this study, we sought to determine the effects and optimal concentrations of antioxidants for the development of porcine embryos in an IVC system. Specifically, we examined the synergistic effects of antioxidants on development to the blastocyst stage in a culture system supplemented with L-cysteine during IVM. Of the antioxidants tested (melatonin, glutathione (GSH), β-mercaptoethanol (β-ME), N-acetylcysteine (NAC) and dithiothreitol (DTT)), addition of GSH (1 mM) or β-ME (25 μM) significantly increased development to the blastocyst stage compared with the controls without antioxidant treatment (22.2 ± 4.2% for 1 mM GSH, 25.9 ± 2.2% for 25 μM β-ME and 12-13% for the control, P<0.05). In addition, the mean cell number per blastocyst was increased by approximately 1.7-fold in the presence of GSH or β -ME. These GSH- and β-ME-induced increases in development to the blastocyst stage and total cell number, however, were not mimicked by melatonin, NAC or DTT, all of which are ROS scavengers. The combination of GSH or β-ME with L-cysteine significantly reduced high O2 tension-induced ROS production (P<0.05). These results suggest that a combination of 1 mM GSH or 25 μM β-ME with 1 mM L-cysteine could be used for production of high quality porcine blastocysts in IVC systems.<br>

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