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Selection of viable <i>in vitro</i>-fertilized bovine embryos using time-lapse monitoring in microwell culture dishes
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- SUGIMURA Satoshi
- Department of Biological Production, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
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- AKAI Tomonori
- Dai Nippon Printing Co., Ltd., Kashiwa, Chiba 277-0871, Japan
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- IMAI Kei
- Department of Sustainable Agriculture, Rakuno Gakuen University, Hokkaido 069-8501, Japan
Bibliographic Information
- Other Title
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- Selection of viable in vitro-fertilized bovine embryos using time-lapse monitoring in microwell culture dishes
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Description
<p> Conventionally, in vitro-fertilized (IVF) bovine embryos for transfer are morphologically evaluated at day 7–8 of embryo culture. This method is, however, subjective and results in unreliable selection. We previously described a novel selection system for IVF bovine blastocysts for transfer that traces the development of individual embryos with time-lapse monitoring in our specially developed microwell culture dishes (LinKID micro25). The system can noninvasively identify prognostic factors that reflect viability after transfer. By assessing a combination of identified prognostic factors —timing of the first cleavage; number of blastomeres at the end of the first cleavage; and number of blastomeres at the onset of lag-phase, which results in temporary developmental arrest during the fourth or fifth cell cycle— the pregnancy rate was improved over using conventional morphological evaluation. Time-lapse monitoring with LinKID micro25 could facilitate objective and reliable selection of healthy IVF bovine embryos. Here, we review the novel bovine embryo selection system that allows for prediction of viability after transfer.</p>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 63 (4), 353-357, 2017
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390001206334994432
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- NII Article ID
- 130005982811
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- HANDLE
- 10659/00006057
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- NDL BIB ID
- 028458021
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- PubMed
- 28552887
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed